POS0457 LABIAL AND PAROTID SALIVARY GLAND HISTOPATHOLOGY IN PRIMARY SJÖGREN’S SYNDROME

Background Salivary gland involvement is a hallmark of disease in primary Sjögren’s syndrome (pSS). This reflected by the prominent role positive biopsy within ACR-EULAR classification criteria, which solely based on focus score (FS) 1 . In pSS all salivary glands might be involved process. For this reason, both labial and parotid biopsies can used for diagnosis evaluation. Although nearly clinical centres obtain pSS, also safe effective procedure. Furthermore, it has comparable diagnostic potential associated with less morbidity, may even detect presence subclinical MALT lymphomas 2 However, histopathological differences between types should potentially taken into account. Objectives The aim study was to get insight minor (labial) major (parotid) patients comparison non-SS sicca patients. Methods Both were obtained from 99 According expert opinion three experienced rheumatologists, 36 classified as 63 formalin fixed, paraffine embedded serially sectioned at 3-4µm. Sections stained H&E CD3, CD20, CD45, hmwCK, CD21, Bcl6, IgA/IgG IgM. Focus (FS), relative area lymphocytic infiltrate, level lymphoid organization plasma cell shift determined. addition, number lymphoepithelial lesions, germinal centers, follicular dendritic networks, B-cells T-cells analyzed. Results All parameters differed significantly sections. Comparison two revealed more signs inflammation shown higher FS, CD3 + T-cells, CD20 CD45 infiltrates compared biopsies. Other glands. patients, FS observed Nevertheless, absolute B-cell counts, GCs/mm LELs/mm parenchyma. Conclusion shows (unspecific) hyperactivity, such B-cells, , are pronounced, These consideration disease. References [1]Shiboski CH, Shiboski SC, Seror R, et al. 2016 American College Rheumatology/European League Against Rheumatism Classification Criteria Primary Syndrome: A Consensus Data-Driven Methodology Involving Three International Patient Cohorts. Arthritis Rheumatol 2017. doi:10.1002/art.39859 [2]Pijpe J, Vissink A, Kallenberg CGM, Spijkervet FKL. Parotid syndrome: Reply [8]. Rheumatology 2007;46(2):371. doi:10.1093/rheumatology/kel361 financially supported grant National Institutes Dental Craniofacial Research (U01 DE017593 Biomarkers Syndrome Detection-Definitive Validation). Disclosure Interests None declared.